ABSTRACT
<p><b>OBJECTIVE</b>To investigate the target cells and molecules with sodium valproate induced differentiation of human glioma cells.</p><p><b>METHODS</b>Nude mice bearing human glioma xenogenic graft subcutaneously were treated with sodium valproate. The expressions of HDAC1 and Tob genes of xenografts were analyzed with semiquantitative RT-PCR. The CD133+ cells (BTSCs) were isolated from glioma specimens by immunomagnetic sorting, and cultured in the medium containing FCS or in the serum-free medium supplemented with growth factors, respectively, followed by treatment with sodium valproate in vitro for 21 days. The cell surface markers were detected with flow cytometry and confocal microscopy.</p><p><b>RESULTS</b>Sodium valproate inhibited the growth of subcutaneous xenografs bearing on nude mice (P<0.05), and up-regulated the HDAC1 expression (P<0.01), down-regulated the Tob expression (P<0.05). The cell surface markers of BTSCs were detected by flow cytometry after sodium valproate treatment for 21 days. In the FCS group, the GFAP or beta-tubulin III positive cells increased significantly (P<0.01), but in the growth factor group, no statistical differences were observed in the GFAP or beta-tubulin III expression (P>0.05). The results of confocal microscopy indicated that the GFAP+ or beta-tubulin III+ cells coexpressed with Nestin.</p><p><b>CONCLUSIONS</b>HDAC1 and Tob genes were the potential target molecules in reversion of the differential inhibition of human glioma cells with sodium valproate. The BTSCs undergoing the processes of differentiation were the target cells for sodium valproate.</p>
Subject(s)
Animals , Humans , Mice , AC133 Antigen , Actins , Antigens, CD , Brain Neoplasms , Metabolism , Pathology , Cell Differentiation , Flow Cytometry , Gene Expression , Glial Fibrillary Acidic Protein , Glioma , Metabolism , Pathology , Glycoproteins , Histone Deacetylases , Genetics , Intermediate Filament Proteins , Mice, Nude , Microscopy, Confocal , Nerve Tissue Proteins , Nestin , Peptides , Reverse Transcriptase Polymerase Chain Reaction , Tumor Cells, Cultured , Tumor Suppressor Proteins , Metabolism , Valproic Acid , Pharmacology , Xenograft Model Antitumor Assays , MethodsABSTRACT
<p><b>OBJECTIVE</b>To isolate and culture tumor stem cells from glioma tissues obtained at surgical operation and to study their biological characteristics.</p><p><b>METHODS</b>Glioma tissues obtained from surgically resected specimens of 8 patients were fully chopped, trypsinized, and filtered to prepare single cell suspensions. The cells were cultured in serum-free medium with EGF, LIF and bFGF. CD133(+) cells were purified by magnetic cell sorting, and cultured continuously in vitro to obtain tumor cell spheres. Tumor stem cells of the 5th passage were induced to differentiate with 10% FBS, and expression of cell differentiation markers such as Nestin, MAP2, GFAP was evaluated with immunocytochemistry techniques.</p><p><b>RESULTS</b>CD133(+) cells were successfully separated and cultured from one anasplastic mixed astrocyte-ependymocyte type glioma specimen. These cells maintained a sphere-like growth status in vitro (3 months, 14 passages), and can self-renew, proliferate and conditionally differentiate into MAP2(+) and GFAP(+) cells. However, CD133(-) cells did not possess these properties.</p><p><b>CONCLUSION</b>Glioma tissue contains tumor stem cells. Those cells can be cultured and passaged in vitro for a long term, and therefore to offer new approaches for studying cellular and molecular biology of glioma.</p>